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Appl Environ Microbiol. 2014 Oct;80(20):6328-33. doi: 10.1128/AEM.01036-14. Epub 2014 Aug 8.

Vibrio harveyi adheres to and penetrates tissues of the European abalone Haliotis tuberculata within the first hours of contact.

Author information

1
UMR 6539-Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), Université de Bretagne Occidentale (UBO), CNRS, IRD, Ifremer, Technopôle Brest Iroise, Plouzané, France marion.cardinaud@gmail.com christine.paillard@univ-brest.fr.
2
UMR 6539-Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), Université de Bretagne Occidentale (UBO), CNRS, IRD, Ifremer, Technopôle Brest Iroise, Plouzané, France.

Abstract

Vibrio harveyi is a marine bacterial pathogen responsible for episodic epidemics generally associated with massive mortalities in many marine organisms, including the European abalone Haliotis tuberculata. The aim of this study was to identify the portal of entry and the dynamics of infection of V. harveyi in the European abalone. The results indicate that the duration of contact between V. harveyi and the European abalone influences the mortality rate and precocity. Immediately after contact, the epithelial and mucosal area situated between the gills and the hypobranchial gland was colonized by V. harveyi. Real-time PCR analyses and culture quantification of a green fluorescent protein-tagged strain of V. harveyi in abalone tissues revealed a high density of bacteria adhering to and then penetrating the whole gill-hypobranchial gland tissue after 1 h of contact. V. harveyi was also detected in the hemolymph of a significant number of European abalones after 3 h of contact. In conclusion, this article shows that a TaqMan real-time PCR assay is a powerful and useful technique for the detection of a marine pathogen such as V. harveyi in mollusk tissue and for the study of its infection dynamics. Thus, we have revealed that the adhesion and then the penetration of V. harveyi in European abalone organs begin in the first hours of contact. We also hypothesize that the portal of entry of V. harveyi in the European abalone is the area situated between the gills and the hypobranchial gland.

PMID:
25107972
PMCID:
PMC4178649
DOI:
10.1128/AEM.01036-14
[Indexed for MEDLINE]
Free PMC Article

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