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Cold Spring Harb Perspect Biol. 2014 Aug 7;6(9):a016428. doi: 10.1101/cshperspect.a016428.

Sources of DNA double-strand breaks and models of recombinational DNA repair.

Author information

1
Rosenstiel Basic Medical Sciences Research Center, MS029 Rosenstiel Center, Brandeis University, Waltham, Massachusetts 02454-9110.

Abstract

DNA is subject to many endogenous and exogenous insults that impair DNA replication and proper chromosome segregation. DNA double-strand breaks (DSBs) are one of the most toxic of these lesions and must be repaired to preserve chromosomal integrity. Eukaryotes are equipped with several different, but related, repair mechanisms involving homologous recombination, including single-strand annealing, gene conversion, and break-induced replication. In this review, we highlight the chief sources of DSBs and crucial requirements for each of these repair processes, as well as the methods to identify and study intermediate steps in DSB repair by homologous recombination.

PMID:
25104768
PMCID:
PMC4142968
DOI:
10.1101/cshperspect.a016428
[Indexed for MEDLINE]
Free PMC Article

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