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J Biosci Bioeng. 2015 Feb;119(2):176-9. doi: 10.1016/j.jbiosc.2014.07.006. Epub 2014 Aug 4.

Enhancement of L-cysteine production by disruption of yciW in Escherichia coli.

Author information

1
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
2
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan. Electronic address: iohtsu@bs.naist.jp.
3
Research Institute for Bioscience Products and Fine Chemicals, Ajinomoto Co., 1-1 Suzuki-cho, Kawasaki, Kanagawa 210-8681, Japan.
4
Faculty of Agriculture, Shinshu University, 8304 Minamiminowa, Nagano 399-4511, Japan; JST, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.

Abstract

Using in silico analysis, the yciW gene of Escherichia coli was identified as a novel L-cysteine regulon that may be regulated by the transcriptional activator CysB for sulfur metabolic genes. We found that overexpression of yciW conferred tolerance to L-cysteine, but disruption of yciW increased L-cysteine production in E. coli.

KEYWORDS:

CysB-binding motif; Escherichia coli; In silico analysis; l-Cysteine fermentation; yciW gene

PMID:
25103863
DOI:
10.1016/j.jbiosc.2014.07.006
[Indexed for MEDLINE]

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