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Nanomedicine. 2014 Nov;10(8):1871-5. doi: 10.1016/j.nano.2014.07.011. Epub 2014 Aug 5.

Nano-topology guided neurite outgrowth in PC12 cells is mediated by miRNAs.

Author information

1
Advanced Materials for Micro- and Nano-Systems Programme, Singapore-MIT Alliance, Singapore.
2
Department of Biochemistry, National University of Singapore, Singapore.
3
Department of Electrical and Computer Engineering, National University of Singapore, Singapore.
4
NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore.
5
Department of Biochemistry, National University of Singapore, Singapore. Electronic address: heng-phon_too@nuhs.edu.sg.
6
Advanced Materials for Micro- and Nano-Systems Programme, Singapore-MIT Alliance, Singapore; Department of Electrical and Computer Engineering, National University of Singapore, Singapore; NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore. Electronic address: elechoi@nus.edu.sg.

Abstract

MicroRNAs (miRNAs) are master regulators of gene expression at post-transcriptional level. The present study investigated the involvement of miRNAs in topological guidance of neurite outgrowth in an NGF treated PC12 cell model cultured on nano-patterned polyethylene terephthalate (PET) substrates fabricated with interference lithography. The expressions of 38 neuronal miRNAs were measured and 3 were found to be differentially regulated during topological guidance of neurite outgrowth. Altering the intracellular levels of these miRNAs disrupted the orderly growth of neurite along nano-patterned substrate. Our results showed miRNAs to be versatile regulators and their involvement should be thoroughly investigated for better understanding of biological processes.

FROM THE CLINICAL EDITOR:

In this basic science study, strong evidence was found that topological guidance is only one factor, and miRNA-s regulate axonal outgrowth from neurites. Nano-patterned polyethylene terephthalate substrates were used for the study, fabricated using interference lithography. Further studies of this biologically relevant process may pave the way to clinically useful axonal regrowth and axonal guidance methods.

KEYWORDS:

MicroRNAs; Nano-topology; Neurite; PC12

PMID:
25101881
DOI:
10.1016/j.nano.2014.07.011
[Indexed for MEDLINE]

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