Complete mitochondrial genome of Sebastes vulpes (Scorpaenidae, Scorpaeniformes) from the East Sea, Korea

Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1239-40. doi: 10.3109/19401736.2014.945536. Epub 2014 Aug 7.

Abstract

Sebastes vulpes is considered a valid species despite the presence of extensive hybrids. To obtain the basic genetic intraspecific structure, we analyzed the complete mitogenome of S. vulpes using next-generation sequencing. The complete mitogenome was 16,462 bp in length containing 13 protein-coding genes (PCGs), 2 rRNAs, 22 tRNAs and 1 control region. The complete mitogenome comprised 27.7% A, 26.5% T, 17.1% G and 28.6% C, showing a minor AT bias (54.3%). The initial codon in all PCGs was ATG, excluding COX1 (GTG). The majority of stop codons in the PCGs were TAA, excluding ND1 and ND3 (TAG), ND4 (AGA) and Cytb (incomplete termination codon, T). All tRNAs had a typical cloverleaf structure, excluding tRNA(Ser (AGY)), which lacked the DHU arm. The complete mitogenome of S. vulpes can be used to study hybridisation and on-going speciation.

Keywords: Mitochondrial genome; Sebastes vulpes; Sebastinae; rockfish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Composition / genetics
  • Base Sequence
  • Codon, Initiator / genetics
  • Codon, Terminator / genetics
  • DNA, Mitochondrial / genetics*
  • Genome Size / genetics
  • Genome, Mitochondrial / genetics*
  • Mitochondria / genetics*
  • Perciformes / genetics*
  • Polymerase Chain Reaction / veterinary
  • RNA, Ribosomal / genetics
  • RNA, Transfer / genetics
  • Republic of Korea
  • Sequence Analysis, DNA / veterinary*

Substances

  • Codon, Initiator
  • Codon, Terminator
  • DNA, Mitochondrial
  • RNA, Ribosomal
  • RNA, Transfer