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FEBS Lett. 2014 Sep 17;588(18):3352-60. doi: 10.1016/j.febslet.2014.07.024. Epub 2014 Aug 4.

The transcription factors Sox5 and Sox9 regulate Catsper1 gene expression.

Author information

1
Unidad de Investigación Médica en Enfermedades Metabólicas, Instituto Mexicano del Seguro Social (IMSS), México DF 06720, Mexico; Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), México DF 07360, Mexico.
2
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), México DF 07360, Mexico.
3
Unidad de Investigación Médica en Enfermedades Metabólicas, Instituto Mexicano del Seguro Social (IMSS), México DF 06720, Mexico.
4
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Cuernavaca 62210, Mexico.
5
Departamento de Biología Celular, Cinvestav, México DF 07360, Mexico.
6
Unidad de Investigación Médica en Enfermedades Metabólicas, Instituto Mexicano del Seguro Social (IMSS), México DF 06720, Mexico. Electronic address: naoviedoa@yahoo.com.mx.

Abstract

Catsper is a Ca(2+)permeable channel required for sperm hyperactivation. In spite of its central role in male fertility, the transcriptional mechanisms that regulate Catsper1 expression are ill defined. In this work, we describe the identification and characterization of important regulatory elements in the murine Catsper1 gene proximal promoter. Four transcription start sites and three functional Sox-binding sites were identified in the Catsper1 promoter. Interestingly, transcription factors Sox5 and Sox9 caused a significant increase in transactivation of the Catsper1 promoter in heterologous systems, and chromatin immunoprecipitation assays showed that both transcription factors interact with the Catsper1 promoter in vivo. These results provide new insights into the molecular mechanisms that control Catsper channel expression.

KEYWORDS:

Calcium channels; Catsper1; Sox5; Sox9; Transcriptional regulation

PMID:
25101494
DOI:
10.1016/j.febslet.2014.07.024
[Indexed for MEDLINE]
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