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Transbound Emerg Dis. 2014 Oct;61(5):397-410. doi: 10.1111/tbed.12269. Epub 2014 Aug 7.

Investigation into the role of potentially contaminated feed as a source of the first-detected outbreaks of porcine epidemic diarrhea in Canada.

Author information

1
Canadian Food Inspection Agency, National Centres for Animal Disease, National Centre for Foreign Animal Disease, Winnipeg, MB, Canada.

Abstract

In January 2014, approximately 9 months following the initial detection of porcine epidemic diarrhea (PED) in the USA, the first case of PED was confirmed in a swine herd in south-western Ontario. A follow-up epidemiological investigation carried out on the initial and 10 subsequent Ontario PED cases pointed to feed as a common risk factor. As a result, several lots of feed and spray-dried porcine plasma (SDPP) used as a feed supplement were tested for the presence of PEDV genome by real-time RT-PCR assay. Several of these tested positive, supporting the notion that contaminated feed may have been responsible for the introduction of PEDV into Canada. These findings led us to conduct a bioassay experiment in which three PEDV-positive SDPP samples (from a single lot) and two PEDV-positive feed samples supplemented with this SDPP were used to orally inoculate 3-week-old piglets. Although the feed-inoculated piglets did not show any significant excretion of PEDV, the SDPP-inoculated piglets shed PEDV at a relatively high level for ≥9 days. Despite the fact that the tested PEDV genome positive feed did not result in obvious piglet infection in our bioassay experiment, contaminated feed cannot be ruled out as a likely source of this introduction in the field where many other variables may play a contributing role.

KEYWORDS:

porcine epidemic diarrhea; spray-dried porcine plasma; transmission

PMID:
25098383
PMCID:
PMC4282400
DOI:
10.1111/tbed.12269
[Indexed for MEDLINE]
Free PMC Article

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