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Clin Proteomics. 2014 Jul 14;11(1):29. doi: 10.1186/1559-0275-11-29. eCollection 2014.

Proteomic analysis of human vitreous humor.

Author information

1
Institute of Bioinformatics, International Technology Park, Bangalore 560 066, India.
2
Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, Kerala 690 525, India.
3
Vittala International Institute Of Ophthalmology, Bangalore, Karnataka 560085, India.
4
Department of Biotechnology, Kuvempu University, Shankaraghatta, Karnataka 577 451, India.
5
Centre of Excellence in Bioinformatics, Bioinformatics Centre, School of Life Sciences, Pondicherry University, Puducherry 605 014, India.
6
Department of Neurochemistry, National Institute of Mental Health and Neuro Sciences, Bangalore 560 006, India.
7
Armed Forces Medical College, Pune 411 040, India.
8
Department of Biological Chemistry, McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore 21205 MD, USA.
9
Department of Oncology and Pathology, Johns Hopkins University School of Medicine, Baltimore 21205 MD, USA.
#
Contributed equally

Abstract

BACKGROUND:

The vitreous humor is a transparent, gelatinous mass whose main constituent is water. It plays an important role in providing metabolic nutrient requirements of the lens, coordinating eye growth and providing support to the retina. It is in close proximity to the retina and reflects many of the changes occurring in this tissue. The biochemical changes occurring in the vitreous could provide a better understanding about the pathophysiological processes that occur in vitreoretinopathy. In this study, we investigated the proteome of normal human vitreous humor using high resolution Fourier transform mass spectrometry.

RESULTS:

The vitreous humor was subjected to multiple fractionation techniques followed by LC-MS/MS analysis. We identified 1,205 proteins, 682 of which have not been described previously in the vitreous humor. Most proteins were localized to the extracellular space (24%), cytoplasm (20%) or plasma membrane (14%). Classification based on molecular function showed that 27% had catalytic activity, 10% structural activity, 10% binding activity, 4% cell and 4% transporter activity. Categorization for biological processes showed 28% participate in metabolism, 20% in cell communication and 13% in cell growth. The data have been deposited to the ProteomeXchange with identifier PXD000957.

CONCLUSION:

This large catalog of vitreous proteins should facilitate biomedical research into pathological conditions of the eye including diabetic retinopathy, retinal detachment and cataract.

KEYWORDS:

Body fluid proteomics; OFFGEL electrophoresis; Protein biomarkers; Proteome discoverer; Retina; SCX chromatography; Secreted proteins

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