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J Cell Sci. 2014 Oct 1;127(Pt 19):4201-12. doi: 10.1242/jcs.150607. Epub 2014 Aug 5.

Tricellulin regulates junctional tension of epithelial cells at tricellular contacts through Cdc42.

Author information

1
Division of Cell Biology, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
2
Laboratory for Morphogenetic Signaling, RIKEN Center for Developmental Biology, Chuo-ku, Kobe 650-0047, Japan.
3
Department of Biology, Faculty of Sciences, Kyushu University, Kyushu, 812-8581, Japan PRESTO, Japan Science and Technology Agency, Saitama 332-0012, Japan.
4
Division of Cell Biology, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan Division of Cerebral Structure, National Institute for Physiological Sciences, Okazaki 444-8787, Japan Department of Physiological Sciences, The Graduate School for Advanced Studies, Okazaki 444-8585, Japan furuse@nips.ac.jp.

Abstract

When the surface view of each epithelial cell is compared with a polygon, its sides correspond to cell-cell junctions, whereas its vertices correspond to tricellular contacts, whose roles in epithelial cell morphogenesis have not been well studied. Here, we show that tricellulin (also known as MARVELD2), which is localized at tricellular contacts, regulates F-actin organization through Cdc42. Tricellulin-knockdown epithelial cells exhibit irregular polygonal shapes with curved cell borders and impaired organization of F-actin fibers around tricellular contacts during cell-cell junction formation. The N-terminal cytoplasmic domain of tricellulin binds to the Cdc42 guanine-nucleotide-exchange factor (GEF) Tuba (also known as DNMBP and ARHGEF36), and activates Cdc42. A tricellulin mutant that lacks the ability to bind Tuba cannot rescue the curved cell border phenotype of tricellulin-knockdown cells. These findings indicate that tricellular contacts play crucial roles in regulating the actomyosin-mediated apical junctional complex tension through the tricellulin-Tuba-Cdc42 system.

KEYWORDS:

Cdc42; Cell–cell junction; Epithelial cell; F-actin; MARVELD2; Tricellulin; Tuba

PMID:
25097232
DOI:
10.1242/jcs.150607
[Indexed for MEDLINE]
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