Format

Send to

Choose Destination
MBio. 2014 Aug 5;5(4):e01401-14. doi: 10.1128/mBio.01401-14.

Do rumen Bacteroidetes utilize an alternative mechanism for cellulose degradation?

Author information

1
Department of Chemistry, Biotechnology, and Food Science, Norwegian University of Life Sciences, N-1432 Aas, Norway.
2
Department of Plant Biology and Biotechnology, University of Copenhagen, Copenhagen, Denmark.
3
Department of Chemistry, Biotechnology, and Food Science, Norwegian University of Life Sciences, N-1432 Aas, Norway phil.pope@nmbu.no.

Abstract

Uncultured and therefore uncharacterized Bacteroidetes lineages are ubiquitous in many natural ecosystems which specialize in lignocellulose degradation. However, their metabolic contribution remains mysterious, as well-studied cultured Bacteroidetes have been shown to degrade only soluble polysaccharides within the human distal gut and herbivore rumen. We have interrogated a reconstructed genome from an uncultured Bacteroidetes phylotype that dominates a switchgrass-associated community within the cow rumen. Importantly, this characterization effort has revealed the first preliminary evidence for polysaccharide utilization locus (PUL)-catalyzed conversion of cellulose. Based on these findings, we propose a further expansion of the PUL paradigm and the saccharolytic capacity of rumen Bacteroidetes species to include cellulose, the most abundant terrestrial polysaccharide on Earth. Moreover, the perspective of a cellulolytic PUL lays the foundation for PULs to be considered an alternative mechanism for cellulose degradation, next to cellulosomes and free-enzyme systems.

PMID:
25096880
PMCID:
PMC4128358
DOI:
10.1128/mBio.01401-14
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center