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BMC Complement Altern Med. 2014 Aug 5;14:286. doi: 10.1186/1472-6882-14-286.

Neuroprotective effects of melittin on hydrogen peroxide-induced apoptotic cell death in neuroblastoma SH-SY5Y cells.

Author information

1
Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Suwon 441-100, Korea. sangmih@korea.kr.

Abstract

BACKGROUND:

Free radicals are involved in neuronal cell death in human neurodegenerative diseases. Since ancient times, honeybee venom has been used in a complementary medicine to treat various diseases and neurologic disorders. Melittin, the main component of honeybee venom, has various biologic effects, including anti-bacterial, anti-viral, and anti-inflammatory activities.

METHODS:

We investigated the neuroprotective effects of melittin against H2O2-induced apoptosis in the human neuroblastoma cell line SH-SY5Y. The neuroprotective effects of melittin on H2O2-induced apoptosis were investigated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylterazolium bromide assay, caspase 3 activity, 4,6-diamidino-2-phenylindole staining, a lactate dehydrogenase release assay, Western blots, and reverse transcription-polymerase chain reaction.

RESULTS:

The H2O2-treated cells had decreased cell viability with apoptotic features and increased production of caspase-3. On the other hand, melittin treatment increased cell viability and decreased apoptotic DNA fragmentation. Melittin attenuated the H2O2-induced decrease in mRNA and protein production of the anti-apoptotic factor Bcl-2. In addition, melittin inhibited both the H2O2-induced mRNA and protein expression of Bax-associated pro-apoptotic factor and caspase-3.

CONCLUSIONS:

These findings suggest that melittin has potential therapeutic effects as an agent for the prevention of neurodegenerative diseases.

PMID:
25091565
PMCID:
PMC4246557
DOI:
10.1186/1472-6882-14-286
[Indexed for MEDLINE]
Free PMC Article

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