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Plant Physiol Biochem. 2014 Oct;83:51-6. doi: 10.1016/j.plaphy.2014.07.007. Epub 2014 Jul 19.

The cowpea RING ubiquitin ligase VuDRIP interacts with transcription factor VuDREB2A for regulating abiotic stress responses.

Author information

1
Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Electronic address: s.ayan@iitg.ernet.in.
2
Department of Life Science and Bioinformatics, Assam University, Silchar 788011, India. Electronic address: drskpanda@gmail.com.
3
Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Electronic address: ls@iitg.ernet.in.

Abstract

Cowpea (Vigna unguiculata L. Walp) is an important grain legume cultivated in drought-prone parts of the world, having higher tolerance to heat and drought than many other crops. The transcription factor, Dehydration-Responsive Element-Binding protein 2A (DREB2A), controls expression of many genes involved in osmotic and heat stress responses of plants. In Arabidopsis, DREB2A-interacting proteins (DRIPs), which function as E3 ubiquitin ligases (EC 6.3.2.19), regulate the stability of DREB2A by targeting it for proteasome-mediated degradation. In this study, we cloned the cowpea ortholog of DRIP (VuDRIP) using PCR based methods. The 1614 bp long VuDRIP mRNA encoded a protein of 433 amino acids having a C3HC4-type Really Interesting New Gene (RING) domain in the N-terminus and a C-terminal conserved region, similar to Arabidopsis DRIP1 and DRIP2. We found VuDRIP up-regulation in response to various abiotic stresses and phytohormones. Using yeast (Saccharomyces cerevisae) two-hybrid analysis, VuDRIP was identified as a VuDREB2A-interacting protein. The results indicate negative regulation of VuDREB2A by ubiquitin ligases in cowpea similar to Arabidopsis along with their other unknown roles in stress and hormone signaling pathways.

KEYWORDS:

Cowpea; DREB2A; DRIP; Drought; Ubiquitin ligase; Yeast two-hybrid

PMID:
25090086
DOI:
10.1016/j.plaphy.2014.07.007
[Indexed for MEDLINE]

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