Format

Send to

Choose Destination
See comment in PubMed Commons below
PLoS One. 2014 Aug 4;9(8):e102949. doi: 10.1371/journal.pone.0102949. eCollection 2014.

Differences in gene expression between mouse and human for dynamically regulated genes in early embryo.

Author information

1
Department of Biosciences and Nutrition; Center for Biosciences, Karolinska Institutet, Huddinge, Sweden.
2
Department of Biosciences and Nutrition; Center for Biosciences, Karolinska Institutet, Huddinge, Sweden; Department of Obstetrics and Gynecology, Karolinska University Hospital, Stockholm, Sweden.
3
Department of Medical Epidemiology and Biostatistics, Science for Life Laboratory, Karolinska Institute, Stockholm, Sweden.
4
Karolinska Institute, Department of Clinical Science, Intervention and Technology, Karolinska University Hospital K57, Stockholm.
5
Department of Biosciences and Nutrition; Center for Biosciences, Karolinska Institutet, Huddinge, Sweden; Molecular Neurology Program, Research Programs Unit, University of Helsinki, and Folkhälsan Institute of Genetics, Helsinki, Finland.

Abstract

Infertility is a worldwide concern that can be treated with in vitro fertilization (IVF). Improvements in IVF and infertility treatment depend largely on better understanding of the molecular mechanisms for human preimplantation development. Several large-scale studies have been conducted to identify gene expression patterns for the first five days of human development, and many functional studies utilize mouse as a model system. We have identified genes of possible importance for this time period by analyzing human microarray data and available data from online databases. We selected 70 candidate genes for human preimplantation development and investigated their expression in the early mouse development from oocyte to the 8-cell stage. Maternally loaded genes expectedly decreased in expression during development both in human and mouse. We discovered that 25 significantly upregulated genes after fertilization in human included 13 genes whose orthologs in mouse behaved differently and mimicked the expression profile of maternally expressed genes. Our findings highlight many significant differences in gene expression patterns during mouse and human preimplantation development. We also describe four cancer-testis antigen families that are also highly expressed in human embryos: PRAME, SSX, GAGE and MAGEA.

PMID:
25089626
PMCID:
PMC4121084
DOI:
10.1371/journal.pone.0102949
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Support Center