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Oral Oncol. 2014 Oct;50(10):991-9. doi: 10.1016/j.oraloncology.2014.06.012. Epub 2014 Jul 30.

Development and characterization of salivary adenoid cystic carcinoma cell line.

Author information

1
The University of Texas, M.D. Anderson Cancer Center, Sections of Head and Neck Pathology, United States.
2
Baylor College of Medicine, Department of Pediatrics, Texas Children's Cancer and Hematology Centers, United States.
3
The Department of Head and Neck Surgery, M.D. Anderson Cancer Center, United States.
4
The University of Texas, M.D. Anderson Cancer Center, Sections of Head and Neck Pathology, United States. Electronic address: anaggar@mdanderson.org.

Abstract

OBJECTIVE:

To develop in vitro adenoid cystic carcinoma cell line as a surrogate for functional studies.

MATERIALS AND METHODS:

Cells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.

RESULTS:

Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature.

CONCLUSION:

We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.

KEYWORDS:

Adenoid cystic carcinoma; Cell line; Salivary gland tumors

[Indexed for MEDLINE]
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