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Nat Methods. 2014 Sep;11(9):927-930. doi: 10.1038/nmeth.3043. Epub 2014 Aug 3.

High-resolution structure determination by continuous-rotation data collection in MicroED.

Author information

1
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn VA, USA.
2
Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
#
Contributed equally

Abstract

MicroED uses very small three-dimensional protein crystals and electron diffraction for structure determination. We present an improved data collection protocol for MicroED called 'continuous rotation'. Microcrystals are continuously rotated during data collection, yielding more accurate data. The method enables data processing with the crystallographic software tool MOSFLM, which resulted in improved resolution for the model protein lysozyme. These improvements are paving the way for the broad implementation and application of MicroED in structural biology.

PMID:
25086503
PMCID:
PMC4149488
DOI:
10.1038/nmeth.3043
[Indexed for MEDLINE]
Free PMC Article

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