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J Colloid Interface Sci. 2014 Oct 15;432:135-43. doi: 10.1016/j.jcis.2014.07.003. Epub 2014 Jul 12.

Carboxybetaine methacrylate oligomer modified nylon for circulating tumor cells capture.

Author information

1
College of Engineering and Applied Sciences, Nanjing University, Nanjing 210093, China.
2
Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing 210008, China.
3
Department of Chemistry, Stony Brook University, Stony Brook, NY 11790, USA.
4
College of Engineering and Applied Sciences, Nanjing University, Nanjing 210093, China. Electronic address: ztnj@nju.edu.cn.
5
The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University, Clinical Cancer Institute of Nanjing University, 321 Zhongshan Road, Nanjing 210008, China.

Abstract

Circulating tumor cells (CTC) capture is one of the most effective approaches in diagnosis and treatment of cancers in the field of personalized cancer medicine. In our study, zwitterionic carboxybetaine methacrylate (CBMA) oligomers were grafted onto nylon via atomic transfer random polymerization (ATRP) which would serve as a novel material for the development of convenient CTC capture interventional medical devices. The chemical, physical and biological properties of pristine and modified nylon surfaces were assessed by Fourier transform infrared spectra, atomic force microscope, water contact angle measurements, X-ray photoelectron spectroscopy, protein adsorption, platelet adhesion, and plasma recalcification time (PRT) determinations, etc. The results, including the significant decrease of proteins adsorption and platelets adhesion, as well as prolonged PRTs demonstrated the extraordinary biocompatibility and blood compatibility of the modified surface. Furthermore, we showed that upon immobilization of anti-epithelial cell adhesion molecular (anti-EpCAM) antibody onto the CBMA moiety, the modified nylon surface can selectively capture EpCAM positive tumor cells from blood with high efficiency, indicating the potential of the modified nylon in the manufacture of convenient interventional CTC capture medical devices.

KEYWORDS:

ATRP; Capturing; Carboxybetaine methacrylate; Circulating tumor cells; Interventional medical devices; Nylon

PMID:
25086387
DOI:
10.1016/j.jcis.2014.07.003
[Indexed for MEDLINE]

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