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Food Microbiol. 2014 Dec;44:128-35. doi: 10.1016/j.fm.2014.05.012. Epub 2014 Jun 2.

Detection of Toxoplasma gondii and Cyclospora cayetanensis in Mytilus galloprovincialis from Izmir Province coast (Turkey) by Real Time PCR/High-Resolution Melting analysis (HRM).

Author information

1
Department of Parasitology, Faculty of Medicine, Dokuz Eylül University, 35340 Izmir, Turkey.
2
Department of Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71121 Foggia, Italy.
3
Department of Veterinary Sciences, University of Pisa, 56120 Pisa, Italy.
4
Department of Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71121 Foggia, Italy. Electronic address: annunziata.giangaspero@unifg.it.

Abstract

To evaluate the presence of Toxoplasma gondii and Cyclospora cayetanensis in edible shellfish, a total of 795 specimens of Mytilus galloprovincialis from 8 different sites on the west coast of Turkey (Bays of Izmir and Mersin) were examined. Fifty-three pooled samples were created and subjected to EvaGreen(®) RealTime PCR assay and HRM analysis for protozoans detection and oocyst quantification. Seven out of 8 sites were contaminated with at least one protozoan species. Twenty-one out of 53 (39.6%) samples tested positive for T. gondii, C. cayetanensis or both pathogens: 26.4% samples positive for C. cayetanensis; 9.4% for T. gondii, and 3.8% for both protozoans. The number of oocysts in positive samples ranged from 6 to 30 for T. gondii and from 3 to 48 for C. cayetanensis. This is the first report of T. gondii Type 1, and C. cayetanensis in M. galloprovincialis, the most consumed shellfish species in Turkey.

KEYWORDS:

Cyclospora cayetanensis; Mytilus galloprovincialis; Public health; RealTime HRM; Toxoplasma gondii Type I

PMID:
25084654
DOI:
10.1016/j.fm.2014.05.012
[Indexed for MEDLINE]

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