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Acta Crystallogr F Struct Biol Commun. 2014 Aug;70(Pt 8):1022-6. doi: 10.1107/S2053230X1401317X. Epub 2014 Jul 23.

The RpfC (Rv1884) atomic structure shows high structural conservation within the resuscitation-promoting factor catalytic domain.

Author information

1
Crystallography, Institute for Structural and Molecular Biology, Department of Biological Sciences, Birkbeck, University of London, Malet Street, London WC1E 7HX, England.
2
Centre de Biochimie Structurale, CNRS UMR 5048, 29 Rue de Navacelles, 34090 Montpellier, France; INSERM U1054, Université Montpellier I, Montpellier, France.
3
Department of Microbial Diseases, UCL-Eastman Dental Institute, University College London, 256 Gray's Inn Road, London WC1X 8LD, England.
4
The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, England.

Abstract

The first structure of the catalytic domain of RpfC (Rv1884), one of the resuscitation-promoting factors (RPFs) from Mycobacterium tuberculosis, is reported. The structure was solved using molecular replacement once the space group had been correctly identified as twinned P21 rather than the apparent C2221 by searching for anomalous scattering sites in P1. The structure displays a very high degree of structural conservation with the previously published structures of the catalytic domains of RpfB (Rv1009) and RpfE (Rv2450). This structural conservation highlights the importance of the versatile domain composition of the RPF family.

KEYWORDS:

Mycobacterium tuberculosis; peptidoglycan; resuscitation-promoting factor

PMID:
25084374
PMCID:
PMC4118796
DOI:
10.1107/S2053230X1401317X
[Indexed for MEDLINE]
Free PMC Article

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