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Metab Eng. 2014 Sep;25:174-82. doi: 10.1016/j.ymben.2014.07.007. Epub 2014 Jul 28.

Metabolic engineering for higher alcohol production.

Author information

1
Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
2
Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA; Microbiology Graduate Group, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
3
Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA; Microbiology Graduate Group, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA. Electronic address: satsumi@ucdavis.edu.

Abstract

Engineering microbial hosts for the production of higher alcohols looks to combine the benefits of renewable biological production with the useful chemical properties of larger alcohols. In this review we outline the array of metabolic engineering strategies employed for the efficient diversion of carbon flux from native biosynthetic pathways to the overproduction of a target alcohol. Strategies for pathway design from amino acid biosynthesis through 2-keto acids, from isoprenoid biosynthesis through pyrophosphate intermediates, from fatty acid biosynthesis and degradation by tailoring chain length specificity, and the use and expansion of natural solvent production pathways will be covered.

KEYWORDS:

Biofuel; Butanol; Higher alcohol; Isobutanol

PMID:
25080238
DOI:
10.1016/j.ymben.2014.07.007
[Indexed for MEDLINE]

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