Whole-genome sequencing of the Mycobacterium tuberculosis Manila sublineage results in less clustering and better resolution than mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing and spoligotyping

F B Jamieson; S Teatero; J L Guthrie; A Neemuchwala; N Fittipaldi; C Mehaffy

doi:10.1128/JCM.01726-14

Whole-genome sequencing of the Mycobacterium tuberculosis Manila sublineage results in less clustering and better resolution than mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing and spoligotyping

J Clin Microbiol. 2014 Oct;52(10):3795-8. doi: 10.1128/JCM.01726-14. Epub 2014 Jul 30.

Authors

F B Jamieson¹, S Teatero², J L Guthrie², A Neemuchwala², N Fittipaldi¹, C Mehaffy³

Affiliations

¹ Public Health Ontario, Toronto, Ontario, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
² Public Health Ontario, Toronto, Ontario, Canada.
³ Public Health Ontario, Toronto, Ontario, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada carolina.mehaffy@colostate.edu.

Abstract

Mycobacterium tuberculosis isolates of the Manila sublineage are genetically homogeneous. In this study, we used whole-genome sequencing (WGS) to type a collection of 36 M. tuberculosis isolates of the Manila family. WGS enabled the subtyping of these 36 isolates into at least 10 distinct clusters. Our results indicate that WGS is a powerful approach to determining the relatedness of Manila family M. tuberculosis isolates.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Cluster Analysis
DNA, Bacterial / chemistry
DNA, Bacterial / genetics*
Genome, Bacterial*
Genotype
Humans
Molecular Epidemiology / methods
Molecular Typing / methods*
Mycobacterium tuberculosis / classification*
Mycobacterium tuberculosis / genetics*
Sequence Analysis, DNA / methods*

Substances

DNA, Bacterial

Associated data

SRA/SRP044223