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Blood Transfus. 2014 Jul;12(3):376-87. doi: 10.2450/2014.0266-13. Epub 2014 Jun 5.

Storing red blood cells with vitamin C and N-acetylcysteine prevents oxidative stress-related lesions: a metabolomics overview.

Author information

1
Department of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy.

Abstract

BACKGROUND:

Recent advances in red blood cell metabolomics have paved the way for further improvements of storage solutions.

MATERIALS AND METHODS:

In the present study, we exploited a validated high performance liquid chromatography-mass spectrometry analytical workflow to determine the effects of vitamin C and N-acetylcysteine supplementation (anti-oxidants) on the metabolome of erythrocytes stored in citrate-phosphate-dextrose saline-adenine-glucose-mannitol medium under blood bank conditions.

RESULTS:

We observed decreased energy metabolism fluxes (glycolysis and pentose phosphate pathway). A tentative explanation of this phenomenon could be related to the observed depression of the uptake of glucose, since glucose and ascorbate are known to compete for the same transporter. Anti-oxidant supplementation was effective in modulating the redox poise, through the promotion of glutathione homeostasis, which resulted in decreased haemolysis and less accumulation of malondialdehyde and oxidation by-products (including oxidized glutathione and prostaglandins).

DISCUSSION:

Anti-oxidants improved storage quality by coping with oxidative stress at the expense of glycolytic metabolism, although reservoirs of high energy phosphate compounds were preserved by reduced cyclic AMP-mediated release of ATP.

PMID:
25074788
PMCID:
PMC4111820
DOI:
10.2450/2014.0266-13
[Indexed for MEDLINE]
Free PMC Article

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