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Mamm Genome. 2014 Oct;25(9-10):522-38. doi: 10.1007/s00335-014-9533-z. Epub 2014 Jul 30.

Approach to assessing determinants of glucose homeostasis in the conscious mouse.

Author information

1
Department of Molecular Physiology and Biophysics, School of Medicine, Vanderbilt University, 823 Light Hall, 2215 Garland Ave, Nashville, TN, 37232, USA, curtis.hughey.1@vanderbilt.edu.

Abstract

Obesity and type 2 diabetes lessen the quality of life of those afflicted and place considerable burden on the healthcare system. Furthermore, the detrimental impact of these pathologies is expected to persist or even worsen. Diabetes is characterized by impaired insulin action and glucose homeostasis. This has led to a rapid increase in the number of mouse models of metabolic disease being used in the basic sciences to assist in facilitating a greater understanding of the metabolic dysregulation associated with obesity and diabetes, the identification of therapeutic targets, and the discovery of effective treatments. This review briefly describes the most frequently utilized models of metabolic disease. A presentation of standard methods and technologies on the horizon for assessing metabolic phenotypes in mice, with particular emphasis on glucose handling and energy balance, is provided. The article also addresses issues related to study design, selection and execution of metabolic tests of glucose metabolism, the presentation of data, and interpretation of results.

PMID:
25074441
PMCID:
PMC4167555
DOI:
10.1007/s00335-014-9533-z
[Indexed for MEDLINE]
Free PMC Article

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