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J Am Chem Soc. 2014 Aug 20;136(33):11582-5. doi: 10.1021/ja505305z. Epub 2014 Aug 7.

Tet-mediated formation of 5-hydroxymethylcytosine in RNA.

Author information

1
Environmental Toxicology Graduate Program and ‡Department of Chemistry, University of California , Riverside, California 92521, United States.

Abstract

Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (∼1 per 5000 5-methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.

PMID:
25073028
PMCID:
PMC4140497
DOI:
10.1021/ja505305z
[Indexed for MEDLINE]
Free PMC Article

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