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J Immunol. 2014 Sep 1;193(5):2207-17. doi: 10.4049/jimmunol.1400411. Epub 2014 Jul 28.

Transcription factor ABF-1 suppresses plasma cell differentiation but facilitates memory B cell formation.

Author information

1
Genomics Research Center, Academia Sinica, Taipei 115, Taiwan; Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, Taiwan;
2
Genomics Research Center, Academia Sinica, Taipei 115, Taiwan; Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei 112, Taiwan; and.
3
Genomics Research Center, Academia Sinica, Taipei 115, Taiwan;
4
Genomics Research Center, Academia Sinica, Taipei 115, Taiwan; Institute of Immunology, National Taiwan University, Taipei 110, Taiwan.
5
Genomics Research Center, Academia Sinica, Taipei 115, Taiwan; Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, Taiwan; Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei 112, Taiwan; and Institute of Immunology, National Taiwan University, Taipei 110, Taiwan kuoilin@gate.sinica.edu.tw.

Abstract

Ag-primed B cells that result from an immune response can form either memory B cells or Ab-secreting plasma cells; however, the molecular machinery that controls this cellular fate is poorly understood. In this study, we show that activated B cell factor-1 (ABF-1), which encodes a basic helix-loop-helix transcriptional repressor, participates in this regulation. ABF-1 was prevalently expressed in purified memory B cells and induced by T follicular helper cell-mediated signals. ABF-1 expression declined by the direct repression of B lymphocyte-induced maturation protein-1 during differentiation. Ectopic expression of ABF-1 reduced the formation of Ab-secreting cells in an in vitro differentiation system of human memory B cells. Accordingly, knockdown of ABF-1 potentiates the formation of Ab-secreting cells. A transgenic mouse that expresses inducible ABF-1 in a B cell-specific manner was generated to demonstrate that the formation of germinal center and memory B cells was augmented by induced ABF-1 in an immune response, whereas the Ag-specific plasma cell response was dampened. This effect was associated with the ability of ABF-1 to limit cell proliferation. Together, our results demonstrate that ABF-1 facilitates formation of memory B cells but prevents plasma cell differentiation.

PMID:
25070843
DOI:
10.4049/jimmunol.1400411
[Indexed for MEDLINE]
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