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J Med Chem. 2014 Aug 28;57(16):7006-15. doi: 10.1021/jm500596j. Epub 2014 Aug 6.

A potent cyclic peptide targeting SPSB2 protein as a potential anti-infective agent.

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1
Medicinal Chemistry, Monash Institute of Pharmaceutical Sciences, Monash University , Parkville 3052, Victoria, Australia.

Abstract

The protein SPSB2 mediates proteosomal degradation of inducible nitric oxide synthase (iNOS). Inhibitors of SPSB2-iNOS interaction may prolong the lifetime of iNOS and thereby enhance the killing of persistent pathogens. We have designed a cyclic peptide, Ac-c[CVDINNNC]-NH2, containing the key sequence motif mediating the SPSB2-iNOS interaction, which binds to the iNOS binding site on SPSB2 with a Kd of 4.4 nM, as shown by SPR, [(1)H,(15)N]-HSQC, and (19)F NMR. An in vitro assay on macrophage cell lysates showed complete inhibition of SPSB2-iNOS interactions by the cyclic peptide. Furthermore, its solution structure closely matched (backbone rmsd 1.21 Å) that of the SPSB2-bound linear DINNN peptide. The designed peptide was resistant to degradation by the proteases pepsin, trypsin, and chymotrypsin and stable in human plasma. This cyclic peptide exemplifies potentially a new class of anti-infective agents that acts on the host innate response, thereby avoiding the development of pathogen resistance.

PMID:
25068993
DOI:
10.1021/jm500596j
[Indexed for MEDLINE]

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