Systemic gene silencing in plants triggered by fluorescent nanoparticle-delivered double-stranded RNA

Nanoscale. 2014 Sep 7;6(17):9965-9. doi: 10.1039/c4nr03481c.

Abstract

A cationic fluorescence nanoparticle efficiently enters plants with high transfection efficacy. Applying a mixture of G2/dsRNA to the model plant, Arabidopsis root, leads to significant reduction in the expression of important developmental genes and results in apparent phenotypes. This study reports a non-viral gene nanocarrier which triggers gene silencing in plants and leads to systemic phenotypes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / cytology
  • Arabidopsis / genetics*
  • Fluorescent Dyes / chemistry*
  • Materials Testing
  • Nanocapsules / administration & dosage
  • Nanocapsules / chemistry*
  • Nanocapsules / ultrastructure
  • Particle Size
  • Plants, Genetically Modified / genetics
  • RNA Interference*
  • RNA, Double-Stranded / administration & dosage
  • RNA, Double-Stranded / genetics*
  • Surface Properties
  • Transfection / methods*

Substances

  • Fluorescent Dyes
  • Nanocapsules
  • RNA, Double-Stranded