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Stem Cell Reports. 2014 Jun 19;3(1):85-100. doi: 10.1016/j.stemcr.2014.05.011. eCollection 2014 Jul 8.

Direct genesis of functional rodent and human schwann cells from skin mesenchymal precursors.

Author information

1
Program in Neuroscience and Mental Health, Hospital for Sick Children, Toronto, ON M5G 1L7, Canada ; Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, ON M5G 1L7, Canada.
2
Program in Neuroscience and Mental Health, Hospital for Sick Children, Toronto, ON M5G 1L7, Canada ; Institute of Medical Science, University of Toronto, Toronto, ON M5G 0A4, Canada.
3
Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, ON M5G 1L7, Canada.
4
Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 7632700, Israel.
5
Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, ON M5G 1L7, Canada ; Department of Physiology, University of Toronto, Toronto, ON M5G 0A4, Canada ; Department of Surgery, University of Toronto, Toronto, ON M5G 0A4, Canada.
6
Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, ON M5G 1L7, Canada ; Department of Surgery, University of Toronto, Toronto, ON M5G 0A4, Canada.
7
Program in Neuroscience and Mental Health, Hospital for Sick Children, Toronto, ON M5G 1L7, Canada ; Institute of Medical Science, University of Toronto, Toronto, ON M5G 0A4, Canada ; Department of Molecular Genetics, University of Toronto, Toronto, ON M5G 0A4, Canada.
8
Program in Neuroscience and Mental Health, Hospital for Sick Children, Toronto, ON M5G 1L7, Canada ; Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, ON M5G 1L7, Canada ; Institute of Medical Science, University of Toronto, Toronto, ON M5G 0A4, Canada ; Department of Molecular Genetics, University of Toronto, Toronto, ON M5G 0A4, Canada ; Department of Physiology, University of Toronto, Toronto, ON M5G 0A4, Canada.

Abstract

Recent reports of directed reprogramming have raised questions about the stability of cell lineages. Here, we have addressed this issue, focusing upon skin-derived precursors (SKPs), a dermally derived precursor cell. We show by lineage tracing that murine SKPs from dorsal skin originate from mesenchymal and not neural crest-derived cells. These mesenchymally derived SKPs can, without genetic manipulation, generate functional Schwann cells, a neural crest cell type, and are highly similar at the transcriptional level to Schwann cells isolated from the peripheral nerve. This is not a mouse-specific phenomenon, since human SKPs that are highly similar at the transcriptome level can be made from neural crest-derived facial and mesodermally derived foreskin dermis and the foreskin SKPs can make myelinating Schwann cells. Thus, nonneural crest-derived mesenchymal precursors can differentiate into bona fide peripheral glia in the absence of genetic manipulation, suggesting that developmentally defined lineage boundaries are more flexible than widely thought.

PMID:
25068124
PMCID:
PMC4110792
DOI:
10.1016/j.stemcr.2014.05.011
[Indexed for MEDLINE]
Free PMC Article

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