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Gene. 2014 Oct 10;549(2):214-22. doi: 10.1016/j.gene.2014.07.058. Epub 2014 Jul 25.

Cucumis sativus L-type lectin receptor kinase (CsLecRK) gene family response to Phytophthora melonis, Phytophthora capsici and water immersion in disease resistant and susceptible cucumber cultivars.

Author information

1
Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, China.
2
Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, China. Electronic address: 214333407@qq.com.
3
Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China. Electronic address: linyue200@yahoo.com.cn.

Abstract

L-type lectin receptor kinase (LecRK) proteins are an important family involved in diverse biological processes such as pollen development, senescence, wounding, salinity and especially in innate immunity in model plants such as Arabidopsis and tobacco. Till date, LecRK proteins or genes of cucumber have not been reported. In this study, a total of 25 LecRK genes were identified in the cucumber genome, unequally distributed across its seven chromosomes. According to similarity comparison of their encoded proteins, the Cucumis sativus LecRK (CsLecRK) genes were classified into six major clades (from Clade I to CladeVI). Expression of CsLecRK genes were tested using QRT-PCR method and the results showed that 25 CsLecRK genes exhibited different responses to abiotic (water immersion) and biotic (Phytophthora melonis and Phytophthora capsici inoculation) stresses, as well as that between disease resistant cultivar (JSH) and disease susceptible cultivar (B80). Among the 25 CsLecRK genes, we found CsLecRK6.1 was especially induced by P. melonis and P. capsici in JSH plants. All these results suggested that CsLecRK genes may play important roles in biotic and abiotic stresses.

KEYWORDS:

CsLecRK genes; Cucumis sativus; L-type lectin; Phytophthora capsici; Phytophthora melonis; QRT-PCR

PMID:
25065921
DOI:
10.1016/j.gene.2014.07.058
[Indexed for MEDLINE]

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