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Methods Enzymol. 2014;545:265-302. doi: 10.1016/B978-0-12-801430-1.00011-1.

Multidimensional profiling in the investigation of small-molecule-induced cell death.

Author information

1
Residency Program in Pediatrics, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
2
Department of Biological Sciences, Columbia University, New York, USA; Department of Chemistry, Columbia University, New York, USA; Howard Hughes Medical Institute, Columbia University, New York, USA. Electronic address: bstockwell@columbia.edu.

Abstract

Numerous morphological variations of cell death have been described. These processes depend on a complex and overlapping cellular signaling network, making molecular definition of the pathways challenging. This review describes one solution to this problem for small-molecule-induced death, the creation of high-dimensionality profiles for compounds that can be used to define and compare pathways. Such profiles have been assembled from gene expression measurements, protein quantification, chemical-genetic interactions, chemical combination interactions, cancer cell line sensitivity profiling, quantitative imaging, and modulatory profiling. We discuss the advantages and limitations of these techniques in the study of cell death.

KEYWORDS:

Apoptosis; Cell death; Chemical biology; Gene expression; Nonapoptotic cell death; Profiling; Small molecules

[Indexed for MEDLINE]

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