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Nucleic Acids Res. 2014 Sep;42(15):10161-72. doi: 10.1093/nar/gku682. Epub 2014 Jul 26.

Crucial role of the Rcl1p-Bms1p interaction for yeast pre-ribosomal RNA processing.

Author information

1
Institut Européen de Chimie et Biologie, ARNA laboratory, Université de Bordeaux, F-33607 Pessac, France Institut National de la Santé Et de la Recherche Médicale, INSERM - U869, ARNA laboratory, F-33000 Bordeaux, France.
2
Equipe labellisée Ligue Contre le Cancer, Centre National de la Recherche Scientifique, Laboratoire de Biologie Moléculaire Eucaryote and Université de Toulouse, UPS, F-31062 Toulouse Cedex 9, France.
3
Equipe labellisée Ligue Contre le Cancer, Centre National de la Recherche Scientifique, Laboratoire de Biologie Moléculaire Eucaryote and Université de Toulouse, UPS, F-31062 Toulouse Cedex 9, France anthony.henras@ibcg.biotoul.fr.
4
Institut Européen de Chimie et Biologie, ARNA laboratory, Université de Bordeaux, F-33607 Pessac, France Institut National de la Santé Et de la Recherche Médicale, INSERM - U869, ARNA laboratory, F-33000 Bordeaux, France sebastien.fribourg@inserm.fr.

Abstract

The essential Rcl1p and Bms1p proteins form a complex required for 40S ribosomal subunit maturation. Bms1p is a GTPase and Rcl1p has been proposed to catalyse the endonucleolytic cleavage at site A2 separating the pre-40S and pre-60S maturation pathways. We determined the 2.0 Å crystal structure of Bms1p associated with Rcl1p. We demonstrate that Rcl1p nuclear import depends on Bms1p and that the two proteins are loaded into pre-ribosomes at a similar stage of the maturation pathway and remain present within pre-ribosomes after cleavage at A2. Importantly, GTP binding to Bms1p is not required for the import in the nucleus nor for the incorporation of Rcl1p into pre-ribosomes, but is essential for early pre-rRNA processing. We propose that GTP binding to Bms1p and/or GTP hydrolysis may induce conformational rearrangements within the Bms1p-Rcl1p complex allowing the interaction of Rcl1p with its RNA substrate.

PMID:
25064857
PMCID:
PMC4150785
DOI:
10.1093/nar/gku682
[Indexed for MEDLINE]
Free PMC Article

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