Format

Send to

Choose Destination
See comment in PubMed Commons below
Gene. 2014 Oct 1;549(1):141-8. doi: 10.1016/j.gene.2014.07.057. Epub 2014 Jul 23.

Rgn gene is required for gut cell homeostasis after ingestion of sodium dodecyl sulfate in Drosophila.

Author information

1
College of Life Science, Northeast Forestry University, No. 26 Hexing Road, Xiangfang District, Harbin 150040, PR China.
2
College of Life Science, Northeast Forestry University, No. 26 Hexing Road, Xiangfang District, Harbin 150040, PR China. Electronic address: lhjin2000@hotmail.com.

Abstract

Resistance and resilience constitute the two complementary aspects of epithelial host defenses in Drosophila. Epithelial cell homeostasis is necessary for the recovery of damages caused by stress or infections. However, the genes responsible for gut epithelial homeostasis remain poorly understood. Here, we show that rgn(G4035) mutant flies have higher mortality than wild-type flies after ingestion of sodium dodecyl sulfate (SDS). Excessive melanization and increased necrotic cells in the gut contribute to the reduced survival of rgn(G4035) mutant flies following SDS ingestion. rgn mutant flies have a defect in the replenishment of intestinal stem cells (ISCs) following gut damage. The antimicrobial peptide (AMP) expression is affected in rgn(G4035) mutant fly guts. Together, our study provides evidence that rgn gene is essential for gut cell homeostasis following damage in Drosophila.

KEYWORDS:

7-Aminoactinomycin D; Antimicrobial peptide; Melanization; Mutant; Survival

PMID:
25064488
DOI:
10.1016/j.gene.2014.07.057
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center