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Saudi Pharm J. 2014 Jul;22(3):273-9. doi: 10.1016/j.jsps.2013.06.002. Epub 2013 Jun 24.

Plackett-Burman randomization method for Bacterial Ghosts preparation form E. coli JM109.

Author information

1
Department of Protein Research, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt ; Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
2
Department of Pharmaceutics, Kayyali Chair for Pharmaceutical Industries, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia ; Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.
3
Department of Pharmaceutics, Kayyali Chair for Pharmaceutical Industries, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.

Abstract

Plackett-Burman randomization method is a conventional tool for variables randomization aiming at optimization. Bacterial Ghosts (BGs) preparation has been recently established using methods other than the E lysis gene. The protocol has been based mainly on using critical concentrations from chemical compounds able to convert viable cells to BGs. The Minimum Inhibition Concentration (MIC) and the Minimum Growth Concentration (MGC) were the main guide for the BGs preparation. In this study, Escherichia coli JM109 DEC has been used to produce the BGs following the original protocol. The study contained a detail protocol for BGs preparation that could be used as a guide.

KEYWORDS:

Bacterial Ghosts; E. coil JM109; Plackett–Burman

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