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Plant Cell Physiol. 2014 Sep;55(9):1679-89. doi: 10.1093/pcp/pcu099. Epub 2014 Jul 23.

A thaumatin-like protein, Rj4, controls nodule symbiotic specificity in soybean.

Author information

1
National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602 Japan.
2
Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai-Nishi, Miyazaki, Miyazaki, 889-2192 Japan Department of Agriculture and Forest Sciences, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu-cho, Matsue, Shimane, 690-8504 Japan.
3
National Agriculture and Food Research Organization, Kyushu Okinawa Agricultural Research Center, 2421 Suya, Koshi, Kumamoto, 861-1192 Japan.
4
National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602 Japan Department of Life Science, International Christian University, 3-10-2, Osawa, Mitaka, Tokyo, 181-8585 Japan.
5
Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai-Nishi, Miyazaki, Miyazaki, 889-2192 Japan.
6
National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602 Japan umehara@affrc.go.jp.

Abstract

Soybeans exhibit a nitrogen-fixing symbiosis with soil bacteria of the genera Bradyrhizobium and Ensifer/Sinorhizobium in a unique organ, the root nodule. It is well known that nodulation of soybean is controlled by several host genes referred to as Rj (rj) genes. Among these genes, a dominant allele, Rj4, restricts nodulation with specific bacterial strains such as B. elkanii USDA61 and B. japonicum Is-34. These incompatible strains fail to invade the host epidermal cells as revealed by observations using DsRed-labeled bacteria. Here, we describe the molecular identification of the Rj4 gene by using map-based cloning with several mapping populations. The Rj4 gene encoded a thaumatin-like protein (TLP) that belongs to pathogenesis-related (PR) protein family 5. In rj4/rj4 genotype soybeans and wild soybeans, we found six missense mutations and two consecutive amino acid deletions in the rj4 gene as compared with the Rj4 allele. We also found, using hairy root transformation, that the rj4/rj4 genotype soybeans were fully complemented by the expression of the Rj4 gene. Whereas the expression of many TLPs and other PR proteins is induced by biotic/abiotic stress, Rj4 gene expression appears to be constitutive in roots including root nodules.

KEYWORDS:

Host restriction of nodulation; Nodule symbiotic specificity; Rj gene; Root nodule symbiosis; Soybean; Thaumatin-like protein (TLP)

PMID:
25059584
DOI:
10.1093/pcp/pcu099
[Indexed for MEDLINE]
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