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J Struct Funct Genomics. 2014 Sep;15(3):165-71. doi: 10.1007/s10969-014-9187-9. Epub 2014 Jul 24.

A new manual dispensing system for in meso membrane protein crystallization with using a stepping motor-based dispenser.

Author information

1
RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan, hato-m@gsc.riken.jp.

Abstract

A reliable and easy to use manual dispensing system has been developed for the in meso membrane protein crystallization method. The system consists of a stepping motor-based dispenser with a new microsyringe system for dispensing, which allows us to deliver any desired volume of highly viscous lipidic mesophase in the range from ~50 to at least ~200 nl. The average, standard deviation, and coefficient of variation of 20 repeated deliveries of 50 nl cubic phase were comparable to those of a current robotic dispensing. Moreover, the bottom faces of boluses delivered to the glass crystallization plate were reproducibly circular in shape, and their centers were within about 100 μm from the center of the crystallization well. The system was useful for crystallizing membrane and soluble proteins in meso.

PMID:
25056837
PMCID:
PMC4125823
DOI:
10.1007/s10969-014-9187-9
[Indexed for MEDLINE]
Free PMC Article

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