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Proc Natl Acad Sci U S A. 2014 Aug 5;111(31):11257-62. doi: 10.1073/pnas.1401316111. Epub 2014 Jul 22.

Intravital imaging of cardiac function at the single-cell level.

Author information

1
Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114;Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115; and.
2
Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114; cvinegoni@mgh.harvard.edu rweissleder@mgh.harvard.edu.
3
Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114;
4
Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114;Department of Systems Biology, Harvard Medical School, Boston, MA 02115 cvinegoni@mgh.harvard.edu rweissleder@mgh.harvard.edu.

Abstract

Knowledge of cardiomyocyte biology is limited by the lack of methods to interrogate single-cell physiology in vivo. Here we show that contracting myocytes can indeed be imaged with optical microscopy at high temporal and spatial resolution in the beating murine heart, allowing visualization of individual sarcomeres and measurement of the single cardiomyocyte contractile cycle. Collectively, this has been enabled by efficient tissue stabilization, a prospective real-time cardiac gating approach, an image processing algorithm for motion-artifact-free imaging throughout the cardiac cycle, and a fluorescent membrane staining protocol. Quantification of cardiomyocyte contractile function in vivo opens many possibilities for investigating myocardial disease and therapeutic intervention at the cellular level.

KEYWORDS:

cardiovascular imaging; fluorescence; intravital micoscopy; molecular imaging; pacing

PMID:
25053815
PMCID:
PMC4128110
DOI:
10.1073/pnas.1401316111
[Indexed for MEDLINE]
Free PMC Article

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