Simultaneous determination of zearalenone and its derivatives in edible vegetable oil by gel permeation chromatography and gas chromatography-triple quadrupole mass spectrometry

Mingrong Qian; Hu Zhang; Liqin Wu; Nuo Jin; Jianmei Wang; Kezhi Jiang

doi:10.1016/j.foodchem.2014.05.133

Simultaneous determination of zearalenone and its derivatives in edible vegetable oil by gel permeation chromatography and gas chromatography-triple quadrupole mass spectrometry

Food Chem. 2015 Jan 1:166:23-28. doi: 10.1016/j.foodchem.2014.05.133. Epub 2014 Jun 5.

Authors

Mingrong Qian¹, Hu Zhang², Liqin Wu², Nuo Jin³, Jianmei Wang², Kezhi Jiang⁴

Affiliations

¹ MOA Key Laboratory for Pesticide Residue Detection, Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China; State Key Laboratory Breeding Base for Zhejiang Sustainable, Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, China. Electronic address: qianmr@mail.zaas.ac.cn.
² MOA Key Laboratory for Pesticide Residue Detection, Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China; State Key Laboratory Breeding Base for Zhejiang Sustainable, Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
³ School of Bioengineering, East China University of Science and Technology, Shanghai 200237, China.
⁴ Key Laboratory of Organosilicon Chemistry and Material Technology, Hangzhou Normal University, Hangzhou 310012, China.

PMID: 25053023
DOI: 10.1016/j.foodchem.2014.05.133

Abstract

A sensitive gas chromatographic-triple quadrupole mass spectrometric (GC-QqQ MS) analytical method, for the determination of zearalenone and its five derivatives in edible vegetable oil, was developed. After the vegetable oil samples were prepared using gel permeation chromatography, the eluent was collected, evaporated and dried with nitrogen gas. The residue was silylated with N,O-bis-trimethylsilyltrifluoroacetamide, containing 1% trimethylchlorosilane. GC-QqQ MS was performed in the reaction-monitoring mode to confirm and quantify mycotoxins in vegetable oil. The limits of quantitation were 0.03-0.2 μg kg(-1) for the six mycotoxins. The average recoveries, measured at 2, 20 and 200 μg kg(-1), were in the range 80.3-96.5%. Zearalenone was detected in the range 5.2-184.6 μg kg(-1) in nine maize oils and at 40.7 μg kg(-1) in a rapeseed oil from the local market.

Keywords: Gas chromatography–triple quadrupole mass spectrometry; Gel permeation chromatography; Vegetable oil; Zearalanol; Zearalenol; Zearalenone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Gel / methods*
Gas Chromatography-Mass Spectrometry / methods*
Molecular Structure
Mycotoxins / analysis
Plant Oils / chemistry*
Zearalenone / analysis
Zearalenone / chemistry*

Substances

Mycotoxins
Plant Oils
Zearalenone