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J Chromatogr A. 2014 Sep 5;1358:199-207. doi: 10.1016/j.chroma.2014.06.067. Epub 2014 Jun 26.

Preparation and electrochromatographic characterization of new chiral β-cyclodextrin poly(acrylamidopropyl) porous layer open tubular capillary columns.

Author information

1
School of Chemistry, Monash University, Melbourne, VIC 3800, Australia.
2
School of Chemistry, Monash University, Melbourne, VIC 3800, Australia. Electronic address: milton.hearn@monash.edu.

Abstract

A novel chiral stationary phase consisting of an amidopropyltrimethylammonium chloride divinylbenzene (APAT-DVB) polymer containing the chiral selector heptakis(2,3-di-O-acetyl-6-O-sulfo)-β-cyclodextrin (HSβCD) has been employed in porous layer open tubular (PLOT) capillary column format with various conditions evaluated to optimize the polymerization and chiral selector immobilization. Scanning electron microscopy demonstrated a near homogenous longitudinal open path in the column with a polymer film of uniform thickness. IR spectroscopy characterized the functional groups of the polymer and energy-dispersive X-ray spectroscopy provided further evidence of the successful polymer modification with HSβCD. Optimum electrochromatographic separation conditions were elaborated with respect to organic solvent content and pH of the background electrolyte. Colum-to-column and long-term reproducibility was excellent. The effectiveness of the new capillary column was demonstrated with the successful separation of d-and l-aspartic acid, d- and l-tyrosine and d-and l-lysine.

KEYWORDS:

Chiral selector; Chiral stationary phase; Electrochromatography; In situ polymerization; Porous layer open tubular

PMID:
25052223
DOI:
10.1016/j.chroma.2014.06.067
[Indexed for MEDLINE]
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