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Biotechnol Lett. 2014 Nov;36(11):2311-7. doi: 10.1007/s10529-014-1611-5. Epub 2014 Jul 22.

Cloning and starch degradation profile of maltotriose-producing amylases from Streptomyces species.

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1
Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, 657-8501, Japan.

Abstract

The end products from starch hydrolysis by amylases have important applications in various industries. Here, two amylases derived from two Streptomyces species that hydrolyze soluble starch from potato produced maltotriose as the primary maltooligosaccharide product. The genes, annotated as putative glycoside hydrolases, were cloned and expressed in Streptomyces lividans. These amylases displayed hydrolysis activity from pH 3 to 9.5 and were not affected by Ca(2+.) Optimal production of maltotriose was between 20 and 30 °C at pH 6.5. At the optimal temperature, both amylases produced maltotriose-rich end products rather than either maltose or maltotetraose.

PMID:
25048235
DOI:
10.1007/s10529-014-1611-5
[Indexed for MEDLINE]
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