Format

Send to

Choose Destination
Biomaterials. 2014 Oct;35(31):8757-8766. doi: 10.1016/j.biomaterials.2014.06.053. Epub 2014 Jul 18.

A shape-controlled tuneable microgel platform to modulate angiogenic paracrine responses in stem cells.

Author information

1
Regenerative Medicine Institute, National University of Ireland Galway, Galway, Ireland; Network of Excellence for Functional Biomaterials, National University of Ireland Galway, Galway, Ireland.
2
Network of Excellence for Functional Biomaterials, National University of Ireland Galway, Galway, Ireland.
3
Regenerative Medicine Institute, National University of Ireland Galway, Galway, Ireland.
4
Anatomy, National University of Ireland Galway, Galway, Ireland.
5
Network of Excellence for Functional Biomaterials, National University of Ireland Galway, Galway, Ireland. Electronic address: abhay.pandit@nuigalway.ie.

Abstract

Development of cell delivery platforms have been driven based on an empirical cytoprotective design. While cell-matrix and cell-cell interactions that influence biochemical effects beyond survival has been limited and overshadowed in an effort to incrementally improve biomimicking properties of the tissue-engineered constructs. Here we demonstrate fabrication of a shape controlled 3D type-I collagen-based microgel platform that can be tuned to modulate angiogenic paracrine- 'angiocrine' responses of human mesenchymal stem cells (hMSCs). Furthermore, these microgels were characterized as a 3D cell culture tool to assess optimal biological response as a function of cell-matrix and cell-cell interactions. Finally, optimised hMSC embedded microgels were shown to induce vascular repair and functional improvement in vivo in a mouse model of hind-limb ischemia. The approach described here in designing a tuneable cell delivery platform using naturally occurring extracellular matrix molecules highlights the need for highly customised matrices with an array of self-assembling proteins that dictate specific cell function resembling the native tissue of interest for repair.

KEYWORDS:

Angiogenesis; Cell-factories; Customised ECM matrices; Mesenchymal stem cells; Microgels

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center