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J Vis Exp. 2014 Jul 10;(89). doi: 10.3791/51606.

Development of amelogenin-chitosan hydrogel for in vitro enamel regrowth with a dense interface.

Author information

1
Center for Craniofacial Molecular Biology, Herman Ostrow School of Dentistry, University of Southern California.
2
Center for Craniofacial Molecular Biology, Herman Ostrow School of Dentistry, University of Southern California; joldak@usc.edu.

Abstract

Biomimetic enamel reconstruction is a significant topic in material science and dentistry as a novel approach for the treatment of dental caries or erosion. Amelogenin has been proven to be a critical protein for controlling the organized growth of apatite crystals. In this paper, we present a detailed protocol for superficial enamel reconstruction by using a novel amelogenin-chitosan hydrogel. Compared to other conventional treatments, such as topical fluoride and mouthwash, this method not only has the potential to prevent the development of dental caries but also promotes significant and durable enamel restoration. The organized enamel-like microstructure regulated by amelogenin assemblies can significantly improve the mechanical properties of etched enamel, while the dense enamel-restoration interface formed by an in situ regrowth of apatite crystals can improve the effectiveness and durability of restorations. Furthermore, chitosan hydrogel is easy to use and can suppress bacterial infection, which is the major risk factor for the occurrence of dental caries. Therefore, this biocompatible and biodegradable amelogenin-chitosan hydrogel shows promise as a biomaterial for the prevention, restoration, and treatment of defective enamel.

PMID:
25046057
PMCID:
PMC4214252
DOI:
10.3791/51606
[Indexed for MEDLINE]
Free PMC Article

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