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Microb Ecol Health Dis. 2014 Jul 15;25. doi: 10.3402/mehd.v25.23062. eCollection 2014.

Survival and synergistic growth of mixed cultures of bifidobacteria and lactobacilli combined with prebiotic oligosaccharides in a gastrointestinal tract simulator.

Author information

1
Department of Food Processing, Tallinn University of Technology, Tallinn, Estonia.
2
Competence Center of Food and Fermentation Technologies, Tallinn, Estonia.
3
Department of Clinical Microbiology, Labmedicine Skåne, Lund, Sweden.
4
Department of Clinical Microbiology, Labmedicine Skåne, Lund, Sweden ; Danish Innovation Institute, Copenhagen, Denmark.
5
Department of Food Processing, Tallinn University of Technology, Tallinn, Estonia ; Competence Center of Food and Fermentation Technologies, Tallinn, Estonia.

Abstract

BACKGROUND:

Probiotics, especially in combination with non-digestible oligosaccharides, may balance the gut microflora while multistrain preparations may express an improved functionality over single strain cultures. In vitro gastrointestinal models enable to test survival and growth dynamics of mixed strain probiotics in a controlled, replicable manner.

METHODS:

The robustness and compatibility of multistrain probiotics composed of bifidobacteria and lactobacilli combined with mixed prebiotics (galacto-, fructo- and xylo-oligosaccharides or galactooligosaccharides and soluble starch) were studied using a dynamic gastrointestinal tract simulator (GITS). The exposure to acid and bile of the upper gastrointestinal tract was followed by dilution with a continuous decrease of the dilution rate (de-celerostat) to simulate the descending nutrient availability of the large intestine. The bacterial numbers and metabolic products were analyzed and the growth parameters determined.

RESULTS:

The most acid- and bile-resistant strains were Lactobacillus plantarum F44 and L. paracasei F8. Bifidobacterium breve 46 had the highest specific growth rate and, although sensitive to bile exposure, recovered during the dilution phase in most experiments. B. breve 46, L. plantarum F44, and L. paracasei F8 were selected as the most promising strains for further studies.

CONCLUSIONS:

De-celerostat cultivation can be applied to study the mixed bacterial cultures under defined conditions of decreasing nutrient availability to select a compatible set of strains.

KEYWORDS:

Bifidobacterium; Lactobacillus; gastrointestinal tract simulator; mixed culture; prebiotic oligosaccharides

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