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Obesity (Silver Spring). 2014 Oct;22(10):2210-5. doi: 10.1002/oby.20846. Epub 2014 Jul 17.

α-lipoic acid reduces fatty acid esterification and lipogenesis in adipocytes from overweight/obese subjects.

Author information

1
Department of Nutrition, Food Science and Physiology, University of Navarra, Pamplona, Spain.

Abstract

OBJECTIVE:

α-Lipoic acid (α-LA) is a natural occurring antioxidant with beneficial effects on obesity. The aim of this study was to investigate the putative effects of α-LA on triglyceride accumulation and lipogenesis in subcutaneous adipocytes from overweight/obese subjects and to determine the potential mechanisms involved.

METHODS:

Fully differentiated human subcutaneous adipocytes were treated with α-LA (100 and 250 µM) during 24 h for studying triglyceride content, de novo lipogenesis, and levels of key lipogenic enzymes. The involvement of AMP-activated protein kinase (AMPK) activation was also evaluated.

RESULTS:

α-LA down-regulated triglyceride content by inhibiting fatty acid esterification and de novo lipogenesis. These effects were mediated by reduction in fatty acid synthase (FAS), stearoyl-coenzyme A desaturase 1, and diacylglycerol O-acyltransferase 1 protein levels. Interestingly, α-LA increased AMPK and acetyl CoA carboxylase phosphorylation, while the presence of the AMPK inhibitor Compound C reversed the inhibition observed on FAS protein levels.

CONCLUSIONS:

α-LA down-regulates key lipogenic enzymes, inhibiting lipogenesis and reducing triglyceride accumulation through the activation of AMPK signaling pathway in human subcutaneous adipocytes from overweight/obese subjects.

PMID:
25045030
DOI:
10.1002/oby.20846
[Indexed for MEDLINE]
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