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J Mass Spectrom. 2014 Aug;49(8):737-41. doi: 10.1002/jms.3400.

MALDI-MS analysis and imaging of small molecule metabolites with 1,5-diaminonaphthalene (DAN).

Author information

1
Ames Laboratory-US DOE, Ames, IA, 50011, USA; Department of Chemistry, Iowa State University, Ames, IA, 50011, USA.

Abstract

1,5-Diaminonaphthalene (DAN) has previously been reported as an effective matrix for matrix-assisted laser desorption ionization-mass spectrometry of phospholipids. In the current work, we investigate the use of DAN as a matrix for small metabolite analysis in negative ion mode. DAN was found to provide superior ionization to the compared matrices for MW < ~400 Da; however, 9-aminoacridine (9-AA) was found to be superior for a uridine diphosphate standard (MW 566 Da). DAN was also found to provide a more representative profile of a natural phospholipid mixture than 9-AA. Finally, DAN and 9-AA were applied for imaging of metabolites directly from corn leaf sections. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.

KEYWORDS:

1,5-diaminonaphthalene; MALDI; mass spectrometry imaging; matrix; metabolites; small molecules

PMID:
25044901
DOI:
10.1002/jms.3400

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