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Mol Nutr Food Res. 2014 Oct;58(10):2001-13. doi: 10.1002/mnfr.201400269. Epub 2014 Aug 5.

Transcriptome analysis reveals a dynamic and differential transcriptional response to sulforaphane in normal and prostate cancer cells and suggests a role for Sp1 in chemoprevention.

Author information

1
Linus Pauling Institute, Oregon State University, Corvallis, OR, USA; Biological and Population Health Sciences, Oregon State University, Corvallis, OR, USA.

Abstract

SCOPE:

Epidemiological studies provide evidence that consumption of cruciferous vegetables, like broccoli, can reduce the risk of cancer development. Sulforaphane (SFN) is a phytochemical derived from cruciferous vegetables that induces anti-proliferative and pro-apoptotic responses in prostate cancer cells, but not in normal prostate cells. The mechanisms responsible for this cancer-specific cytotoxicity remain unclear.

METHODS AND RESULTS:

We utilized RNA sequencing and determined the transcriptomes of normal prostate epithelial cells, androgen-dependent prostate cancer cells, and androgen-independent prostate cancer cells treated with SFN. SFN treatment dynamically altered gene expression and resulted in distinct transcriptome profiles depending on prostate cell line. SFN also down-regulated the expression of genes that were up-regulated in prostate cancer cells. Network analysis of genes altered by SFN treatment revealed that the transcription factor Specificity protein 1 (Sp1) was present in an average of 90.5% of networks. Sp1 protein was significantly decreased by SFN treatment in prostate cancer cells and Sp1 may be an important mediator of SFN-induced changes in expression.

CONCLUSION:

Overall, the data show that SFN alters gene expression differentially in normal and cancer cells with key targets in chemopreventive processes, making it a promising dietary anti-cancer agent.

KEYWORDS:

Chemoprevention; Prostate cancer; RNA sequencing; Specificity protein 1; Sulforaphane

PMID:
25044704
PMCID:
PMC4184971
DOI:
10.1002/mnfr.201400269
[Indexed for MEDLINE]
Free PMC Article

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