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Luminescence. 2015 Jun;30(4):382-7. doi: 10.1002/bio.2743. Epub 2014 Jul 18.

Design of highly sensitive phosphorescence sensor for determination of procaterol hydrochloride based on inhibition of KClO3 oxidation fluorescein isothiocyanate.

Author information

1
College of Chemistry and Environment, Minnan Normal University, Zhangzhou, 363000, People's Republic of China.

Abstract

Procaterol hydrochloride (Prh) can inhibit KClO3 oxidation of fluorescein isothiocyanate (FITC) to form a non-phosphorescent compound, which causes room temperature phosphorescence (RTP) of FITC in the system to enhance sharply the linear relationship between ∆Ip and the Prh content. Thus, a rapid response and highly sensitive phosphorescence sensor for the determination of Prh has been developed based on the inhibiting effect of Prh on KClO3 oxidation of FITC. This simple, high sensitivity (detection limit (LD) calculated by 3Sb /k was 0.019 fg/spot, sample volume 0.40 µl, corresponding concentration 4.8 × 10(-14) g ml(-1) ) and selective sensor with a wide linear range (0.080-11.20 g/spot) has been applied to detect Prh in blood samples, and the results were consistent with those obtained by high-performance liquid chromatography (HPLC). Simultaneously, the mechanism of the phosphorescence sensor for the detection of Prh was also investigated using infrared spectroscopy.

KEYWORDS:

Detection technique of residues; Fluorescein isothiocyanate; Inhibition effect; Phosphorescence sensor; Procaterol hydrochloride

PMID:
25044504
DOI:
10.1002/bio.2743
[Indexed for MEDLINE]
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