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Nat Methods. 2014 Aug;11(8):817-820. doi: 10.1038/nmeth.3035. Epub 2014 Jul 20.

Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity.

Author information

1
Epigenetics Programme, Babraham Institute, Cambridge, UK.
2
Wellcome Trust Sanger Institute, Cambridge, UK.
3
European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, UK.
4
Bioinformatics Group, Babraham Institute, Cambridge, UK.
5
Centre for Trophoblast Research, University of Cambridge, Cambridge, UK.
#
Contributed equally

Abstract

We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells grown in serum or in 2i medium displayed epigenetic heterogeneity, with '2i-like' cells present in serum culture. Integration of 12 individual mouse oocyte datasets largely recapitulated the whole DNA methylome, which makes scBS-seq a versatile tool to explore DNA methylation in rare cells and heterogeneous populations.

PMID:
25042786
PMCID:
PMC4117646
DOI:
10.1038/nmeth.3035
[Indexed for MEDLINE]
Free PMC Article

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