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Nat Methods. 2014 Aug;11(8):821-4. doi: 10.1038/nmeth.3031. Epub 2014 Jul 20.

Optimized cell transplantation using adult rag2 mutant zebrafish.

Author information

1
1] Molecular Pathology Unit, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Cancer Center, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [4] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA. [5].
2
1] Molecular Pathology Unit, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Cancer Center, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [4] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.
3
Izaak Walton Killam Health Centre, Dalhousie University, Halifax, Nova Scotia, Canada.
4
1] Department of Surgery, Weill Cornell Medical College, New York, New York, USA. [2] Department of Medicine, Weill Cornell Medical College, New York, New York, USA.

Abstract

Cell transplantation into adult zebrafish has lagged behind mouse models owing to the lack of immunocompromised strains. Here we have created rag2(E450fs) mutant zebrafish that have reduced numbers of functional T and B cells but are viable and fecund. Mutant fish engraft muscle, blood stem cells and various cancers. rag2(E450fs) mutant zebrafish are the first immunocompromised zebrafish model that permits robust, long-term engraftment of multiple tissues and cancer.

PMID:
25042784
PMCID:
PMC4294527
DOI:
10.1038/nmeth.3031
[Indexed for MEDLINE]
Free PMC Article

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