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Biosci Biotechnol Biochem. 2014;78(8):1381-3. doi: 10.1080/09168451.2014.925777. Epub 2014 Jul 21.

Structural determination of an N-glycan moiety attached to the prothoracicotropic hormone from the silkmoth Bombyx mori.

Author information

1
a Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Tokyo , Japan.

Abstract

The predominant structure of the N-glycan on the prothoracicotropic hormone (PTTH) isolated from 1.8 million adult heads of silkmoths was determined to be Manα1-6Manβ1-4GlcNAcβ1-4(Fucα1-6)GlcNAc-OH, which is identical to that of the baculovirus-expressed recombinant PTTH. An ecdysis progression assay demonstrated that N-glycosylated PTTH exhibited a slightly higher activity than the recombinant PTTH without N-glycosylation produced by an Escherichia coli expression system.

KEYWORDS:

Bombyx mori; N-glycan, peptide; metamorphosis; prothoracicotropic hormone

PMID:
25040827
DOI:
10.1080/09168451.2014.925777
[Indexed for MEDLINE]
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