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Exp Cell Res. 2014 Nov 15;329(1):170-7. doi: 10.1016/j.yexcr.2014.07.009. Epub 2014 Jul 17.

Tolerating DNA damage during eukaryotic chromosome replication.

Author information

1
Centro de Biología Molecular Severo Ochoa (CSIC/UAM), Cantoblanco, 28049 Madrid, Spain.
2
Centro de Biología Molecular Severo Ochoa (CSIC/UAM), Cantoblanco, 28049 Madrid, Spain. Electronic address: jatercero@cbm.csic.es.

Abstract

In eukaryotes, the evolutionarily conserved RAD6/RAD18 pathway of DNA damage tolerance overcomes unrepaired DNA lesions that interfere with the progression of replication forks, helping to ensure the completion of chromosome replication and the maintenance of genome stability in every cell cycle. This pathway uses two different strategies for damage bypass: translesion DNA synthesis, which is carried out by specialized polymerases that can replicate across the lesions, and DNA damage avoidance, a process that relies on a switch to an undamaged-DNA template for synthesis past the lesion. In this review, we summarise the current knowledge on DNA damage tolerance mechanisms mediated by RAD6/RAD18 that are used by eukaryotic cells to cope with DNA lesions during chromosome replication.

KEYWORDS:

Chromosome replication; DNA damage tolerance; Genome stability

PMID:
25038291
DOI:
10.1016/j.yexcr.2014.07.009
[Indexed for MEDLINE]

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