Format

Send to

Choose Destination
Gene. 2014 Oct 1;549(1):70-6. doi: 10.1016/j.gene.2014.07.047. Epub 2014 Jul 17.

Cloning and functional expression of a cDNA encoding stearoyl-ACP Δ9-desaturase from the endosperm of coconut (Cocos nucifera L.).

Author information

1
Department of Biotechnology, Hainan University, Haikou, Hainan 570228, China.
2
Department of Biotechnology, Hainan University, Haikou, Hainan 570228, China. Electronic address: hainanzyh@hotmail.com.
3
Department of Biotechnology, Hainan University, Haikou, Hainan 570228, China; Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Hainan University, Haikou, Hainan 570228, China. Electronic address: liddfym@hotmail.com.

Abstract

Coconut (Cocos nucifera L.) is an economically tropical fruit tree with special fatty acid compositions. The stearoyl-acyl carrier protein (ACP) desaturase (SAD) plays a key role in the properties of the majority of cellular glycerolipids. In this paper, a full-length cDNA of a stearoyl-acyl carrier protein desaturase, designated CocoFAD, was isolated from cDNA library prepared from the endosperm of coconut (C. nucifera L.). An 1176 bp cDNA from overlapped PCR products containing ORF encoding a 391-amino acid (aa) protein was obtained. The coded protein was virtually identical and shared the homology to other Δ9-desaturase plant sequences (greater than 80% as similarity to that of Elaeis guineensis Jacq). The real-time fluorescent quantitative PCR result indicated that the yield of CocoFAD was the highest in the endosperm of 8-month-old coconut and leaf, and the yield was reduced to 50% of the highest level in the endosperm of 15-month-old coconut. The coding region showed heterologous expression in strain INVSc1 of yeast (Saccharomyces cerevisiae). GC-MS analysis showed that the levels of palmitoleic acid (16:1) and oleic acid (18:1) were improved significantly; meanwhile stearic acid (18:0) was reduced. These results indicated that the plastidial Δ9 desaturase from the endosperm of coconut was involved in the biosynthesis of hexadecenoic acid and octadecenoic acid, which was similar with other plants. These results may be valuable for understanding the mechanism of fatty acid metabolism and the genetic improvement of CocoFAD gene in palm plants in the future.

KEYWORDS:

Coconut (Cocos nucifera L.); Fatty acid desaturase; Function analysis; Saccharomyces cerevisiae

PMID:
25038276
DOI:
10.1016/j.gene.2014.07.047
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center